HPLC Column, HPS Silica, 5um, 2.1mm ID x 100mm Length, 120A. Cogent Brand. 1 EA.
Additional Info:
75001-10P-2 Cogent HPS Silica™ HPLC Column is packed with high‑purity, unmodified silica, making it an excellent choice for normal phase chromatography and USP L3 methods.
Because the silica surface is unbonded and unmodified, it provides strong, direct interactions with polar and hydrophilic compounds, making it ideal for separations involving fats, lipids, and other polar analytes.
This proprietary HPS Silica™ has been widely trusted in HPLC laboratories for many years as a reliable and cost‑effective alternative to widely used silica columns such as Inertsil® and Luna®.
The 5 µm particle size provides a practical balance of efficiency and robustness, allowing moderate flow rates with lower backpressure, making it compatible with standard HPLC systems and routine analytical workflows.
100 mm Length – Balanced Resolution for Normal Phase HPLC
The 100 mm column length provides improved resolving power while maintaining reasonable run times.
Higher resolution than 50 mm columns
Better separation of closely related compounds
Efficient run times for routine analysis
✅ Recommended for applications requiring added resolution without extended run times
2.1 mm ID – Low‑Flow Analytical Format
Designed for 2.1 mm ID analytical systems
Reduced solvent consumption
Ideal for LC‑MS compatible workflows
Maintains sensitivity in low‑dispersion systems
✅ Recommended for laboratories prioritizing solvent savings and LC‑MS compatibility
Flow Rate & System Requirements (2.1 mm ID, 5 µm)
⚠️ Requires low‑flow capable HPLC or UHPLC system
Typical flow rate range: ~0.2 – 0.4 mL/min
Lower backpressure compared to smaller particle columns
Suitable for standard HPLC systems with low‑flow capability
Compatible with UV and MS detection
✅ Important: Ensure system can deliver stable, consistent low‑flow performance
Why Choose HPS Silica™ Columns
High‑Purity Unmodified Silica
Ideal for normal phase chromatography
Strong interaction with polar compounds
USP L3 Compatibility
Designed for compendial and validated methods
Reliable alternative to legacy silica phases
Reproducible Performance
Consistent selectivity across batches
Proven laboratory reliability
Performance Advantages
Lower Backpressure (5 µm Particles)
Enables routine operation on standard HPLC systems
Allows moderate flow rates
Fast Equilibration
Reduces downtime between runs
Effective Polar Retention
Ideal for hydrophilic compounds, fats, and lipids
Typical Applications
Fats and lipid analysis
Normal phase HPLC separations
Polar compound analysis
USP L3 method development and validation
Food, pharmaceutical, and chemical testing
Important Usage Notes
Designed for normal phase chromatography conditions
Use appropriate non‑polar mobile phases
Ensure proper system equilibration for reproducible retention
Follow standard silica handling and storage practices